Ultimate Flexibility for Your Next RNA-Seq Project

RNA Sequencing (RNA-Seq) is a widely adopted technique for biological research. While innovations over the past decade have decreased the cost-per-sample, researchers are required to multiplex hundreds of samples together to achieve those low costs, resulting in operational inefficiency and long turn-around times. The G4 Sequencing Platform addresses these challenges by enabling researchers to generate fast and accurate RNA-Seq results at a low cost without needing to pool large number of samples.

Innovative G4 Design: Delivering Fast and Flexible RNA Sequencing

Founded on the core principles of accuracy, speed, flexibility, and power – G4 is a versatile and ecosystem compatible benchtop sequencer that is well suited for RNA sequencing.

Key capabilities:

A unique flow cell design and scalable capacity allows labs to process 4–32 samples per run across 1 to 4 flow cells at a time and up to 16 independent lanes.
Produces accurate RNA-Seq data highly correlated with the industry standard benchtop sequencer in less than 24 hours.

*Assumes 25M reads and run times are for 1×50 bp and 2×100 bp run configurations, respectively.

G4 Specifications for RNA Sequencing

G4 can process up to 4 flow cells in parallel. The number of reads and sequencing output shown is per flow cell.
¹Assumes 2×100 bp, 50M read sequencing per sample.

²Run time is dependent on read length.

Performance Characterization of the G4 for RNA-Seq

	F2 Flow Cell	F3 Flow Cell
Paired Reads Delivered / Flow Cell	200M	400M
Paired Reads Delivered / Run	800M	1.6B
# Samples / Flow Cell¹	4	8
# Samples / Run¹	16	32
Price / M Paired Read	$3.5	$2.2
Run Time²	16-18 hours	16-18 hours
Quality	>85% bases ≥ Q30	>85% bases ≥ Q30

The RNA-Seq application note highlights the capability for the G4 to plug into existing NGS library preparation workflows and bioinformatic pipelines to produce highly accurate results.

G4 Plug-and-Play Capability

We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.