Rapid Metagenome Sequencing with the G4
Metagenomic next-generation sequencing (mNGS), also known as shotgun sequencing, is considered a “hypothesis-free” approach that enables researchers to identify potential pathogens in a heterogeneous mixture of microbes, through a sensitive and unbiased method. Traditional methods of pathogen identification, such as culture-based methods, can be time-and labor-intensive, resulting in greater delays to prevent spread of diseases caused by specific microbes and advance treatments and therapies. The G4 Sequencing Platform is addressing these challenges by enabling researchers to generate high-quality mNGS results daily with more operational efficiency and lower cost.
Unmatched Speed and Operational Flexibility, Plus Powerful Resolution
The G4 Sequencing Platform delivers high-quality mNGS data for high resolution of complex microbial communities and de novo genome assembly. Unique platform flexibility enables cost-efficient sequencing with unmatched turnaround times.
- Rapid SBS delivers highly accurate reads with single-day turnaround
- Unbiased and reproducible quantification of low and high GC microorganisms
- Plug-and-play compatibility with standard microbial bioinformatics tools
G4 Specifications for Metagenome Sequencing
| F2 Flow Cell | F3 Flow Cell | |
|---|---|---|
| Paired Reads Delivered / Flow Cell | 200M | 400M |
| Paired Reads Delivered / Run | 800M | 1.6B |
| # Samples / Flow Cell1 | 20 | 40 |
| # Samples / Run1 | 80 | 160 |
| Price / M Paired Read | $4 | $2.5 |
| Run Time | <24 hours | <24 hours |
| Quality | >80% bases ≥ Q30 | >80% bases ≥ Q30 |
G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
1Assumes 2×150 read configuration
Performance Evaluation of the G4 for Microbiome Community Analysis
The metagenome sequencing poster highlights the capabilities of G4 to deliver accurate and reproducible species quantitation comparable with the NextSeq 2000, with more speed and added flexibility.
G4 Plug-and-Play Capability
We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.
Talk to an expert about Metagenome Sequencing on the G4 today
Speak to a member of the Singular team to learn more about the available kits and capabilities for metagenome sequencing on the G4.
