Introducing Max Reads
Maximizing throughput and decreasing cost for your single cell sequencing
$1 per Million Reads
3.2 Billion Reads per Run
(800M Paired Reads per Flow Cell)
16 Samples per Run
(4 Samples per Flow Cell)
The Ultimate Sequencing Kit for Single Cell Experiments
- Designed specifically to complement popular 10x Genomics kits
- Delivers high-throughput, low-cost sequencing on G4
- Generates highly-accurate single cell and spatial data
Modular Throughput Built to Match Your Experiment Size
The Max Read Kit boosts the output of the 4 flow cell G4 Sequencing Platform to an unmatched 3.2 billion paired reads per run on the benchtop system. The kits, specifically designed to complement the format of popular 10x Genomics assays, enable users the flexibility to sequence 4, 8, 12, or 16 single cell1 samples per run, each in its own lane.
| 4 Samples | 8 Samples | 12 Samples | 16 Samples | ||
|---|---|---|---|---|---|
| G4 (Max Read) | Flow Cell | 1 x M3 (800M paired reads) |
2 x M3 (1.6B paired reads) |
3 x M3 (2.4B paired reads) |
4 x M3 (3.2B paired reads) |
| Price / Sample | $200 | $200 | $200 | $200 | |
| Run Time (days) | 1 | 1 | 1 | 1 | |
| NextSeq 20002 | Flow Cell | P3 100 cycle (1,200M paired reads) |
P3 + P2 100 cycle (2 runs, 1.6B paired reads) |
2x P3 100 cycle (2 runs, 2.4B paired reads) |
3x P3 100 cycle (3 runs, 3.6B paired reads) |
| Price / Sample | $832 | $598 | $555 | $624 | |
| Run Time (days) | 1 | 2 | 2 | 3 | |
1Assumes 10,000 cells and 20,000 paired reads
2Reflects publicly available list prices
Powering Your Single Cell Needs
G4 is capable of delivering ~2x more single cell samples per year than NextSeq 2000.
Max Read Kit Specifications
| Product1 | Run Time2 | Paired Reads per Flow Cell | $ per M Paired Read | $ per Sample | Quality |
|---|---|---|---|---|---|
| Max Read™ Single Cell and Spatial Gene Expression | ~30 hours | 800M | $1.00 | $200 | >85% bases ≥ Q30 |
| Max Read™ Spatial Gene Expression for FFPE | ~24 hours | 1.2B | $0.67 |
Max Read Kits contain all components needed to prepare and sequence compatible 10x Genomics libraries on G4, including materials for sample preparation and loading, flow cell, reagent cartridge, and Max Read PhiX Control.
1Currently compatible with 10x Genomics Chromium™ 3′ and 5′ Gene Expression assays and Visium™ Spatial Gene Expression. Kits allow for 1 sample per lane.
2Assumes 10,000 cell/sample and 20,000 paired reads/cell for 1 sample per lane.
Max Read Applications
Max Read technology can be customized to many short read counting applications to generate up to 1 Billion paired reads per flow cell.
Contact us for more information on your application.
Small RNA Seq
miRNA is typically 18–25 nt in length and can be sequenced with 1×50 reads. These small, non-coding RNAs can regulate many biological functions.
cfDNA fragment counting
Analysis of abundance of short fragments of cell-free DNA can be informative for detecing fetal aneuploidy, organ rejection, or cancer.
Proteomics and Spatial Tagging
Proteomics and spatial profiling technologies with NGS readouts typically use short DNA tags that can be sequenced and attributed to specific targets.
mRNA Gene Expression
Gene Expression Profiling (GEP) is achieved by counting transcripts in coding genes. Short, single or paired reads are sufficient for accurate GEP.
CRISPR Screens
In pooled CRISPR Screens, the effects of genetically encoded perturbations can be analyzed through counting of short guide RNAs.
