Powerful Whole Exome Sequencing for Variant Discovery
Whole exome sequencing (WES) enables the discovery and assessment of genetic variations linked to rare or complex diseases and is a key tool for the diagnosis of genetic disease, population genome studies, and tumor-normal sequencing protocols used in precision oncology. With high accuracy and unparalleled flexibility, the G4 Platform delivers reliable WES results with industry-leading speed.
Unparalleled Speed, Power and Operational Flexibility
The G4 Sequencing Platform offers unmatched flexibility, while delivering faster results with high accuracy. G4 is a plug-and-play solution, seamlessly integrating into existing sequencing workflows and laboratory ecosystems.
Key capabilities:
- Rapid SBS enables cost-efficient delivery of 6–52 exome samples in less than 24 hours.*
- G4 delivers highly accurate exome data comparable to leading platforms with SNP and INDEL F1 scores at 99% and 95% respectively.
*Assumes 34 Mb at 100x coverage
G4 Specifications for Whole Exome Sequencing
| F2 Flow Cell | F3 Flow Cell | |
|---|---|---|
| Paired Reads Delivered / Flow Cell | 200M | 400M |
| Paired Reads Delivered / Run | 800M | 1.6B |
| # Samples / Flow Cell1 | 6 | 13 |
| # Samples / Run1 | 24 | 52 |
| Price / M Paired Read | $3.5 | $2.2 |
| Run Time | 15-19 hours | 15-19 hours |
| Quality | >85% bases ≥ Q30 | >85% bases ≥ Q30 |
The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
1Assumes 2×100 read configuration
Performance Characterization of the G4 for Whole Exome Sequencing
The whole exome sequencing application note highlights the capability for the G4 to deliver accurate results comparable with industry-leading platforms with more speed and added flexibility.
G4 Plug-and-Play Capability
We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.
Talk to an expert about Whole Exome Sequencing on the G4 today
Speak to a member of the Singular team to learn more about the available kits and capabilities for whole exome sequencing on the G4.
