G4 Support Hub

Support Video Selector

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Workflow

G4 is the ultimate plug-and-play solution designed to seamlessly fit into existing NGS workflows with easy to integrate Singular adapters and indices as well as partnerships with leading library prep providers and bioinformatics providers.

Prepare

Sequence

Analyze

Virtual G4 Demo

Set up a run on the G4, experience the intuitive user interface, or analyze results in our virtual demo environment.

G4 Documentation

Library Preparation Product Options

The G4 Sequencing Platform is compatible with a range of library preparation products and kits across applications. Explore the products, associated protocols, and data from each library prep partner below. If your library prep workflow is not listed here contact us for more information.

10x Genomics

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
Chromium Single Cell Visium Spatial Gene Expression (FFPE and Fresh Frozen)	SG t-UDI Primers Set A [1–24] SG Non-Indexed Library Prep t-Primers	10x Genomics Library Prep	G4™ Single Cell RNA-Sequencing Performance with the 10x Genomics® Chromium Single Cell 3’ Gene Expression Kit
Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 Chromium Next GEM Single Cell 5’ Reagent Kits v2 Visium Spatial Gene Expression Reagent Kits Visium Spatial Gene Expression Reagent Kits for FFPE	Max Read Single Cell and Spatial Gene Expression Max Read Spatial Gene Expression for FFPE	10x Genomics Library Prep	The Max Read™ Kit for High-Throughput Single Cell Sequencing on the G4™

Agilent Technologies

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
SureSelect Exome v8 and XT HS2 Kits	SG Library Compatibility Kit	Adapting Libraries for the G4™—Retaining Original Indices	Targeted Sequencing Using Agilent SureSelect and the G4™ Sequencing Platform

Bio-Rad Laboratories

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
SEQuoia Express Stranded RNA Library Preparation Kit SEQuoia Complete Stranded RNA Library Preparation Kit SEQuoia RiboDepletion Kit, 24 reactions	SG UDI Primers	Bio-Rad SEQuoia Stranded RNA Library Prep	RNA Sequencing on the Singular Genomics G4™ Sequencing Platform Using SEQuoia™ Library Preparation Chemistries

Claret Biosciences

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
SRSLY PicoPlus ForShear, SRSLY NanoPlus	SG UDI Primers SG Non-Indexed Library Prep Primers	Claret SRSLY Library Prep	Sequence SRSLY® Libraries on the Singular Genomics G4™ Platform

Fluent Biosciences

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
PIPseq 3’ RNA kit	Order custom PCR and sequencing primers	Sequencing PIPseq with Singular Genomics Demonstrated Protocol	PIPseq Compatibility with the Singular Genomics G4 Sequencing Platform

IDT

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
IDT Blockers	Not Applicable	Singular Genomics-specific blockers	Whole Exome Sequencing on the G4™
xGen cfDNA & FFPE DNA Library Prep Kit	SG UDI Primers	xGen™ cfDNA & FFPE DNA Library Prep Kit for the Singular Genomics G4™ Sequencing Platform	Low-Copy Number Variant Detection Using xGen™ NGS DNA Library Prep and G4™ Sequencing Platform
xGen Hybridization and Wash Kit with xGen Exome Hyb Panel v2	SG UDI Primers SG Non-Indexed Library Prep Primers	Coming Soon	Whole Exome Sequencing on the G4®
xGen cfDNA kit with xGen Pan Cancer Panel	Not Applicable	Coming Soon	Rapid Targeted Germline and Somatic Variant Detection Using the G4® Sequencing Platform

NEB

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
NEBNext Enzymatic Methyl-seq Kit	SG UDI Primers SG Non-Indexed Library Prep Primers	Coming Soon	Performance Assessment of the Novel G4® Sequencing Platform for Cancer Research
NEBNext Ultra II Directional RNA Library Prep Kit for Illumina RNA Depletion Kit v2 (Human/ Mouse/ Rat)	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers	NEBNext Ultra II Directional RNA Library Prep	RNA Sequencing Using the New England Biolabs (NEB) Ultra ll RNA Library Prep Kit and the G4® Sequencing Platform
NEBNext Ultra II FS DNA Library Prep Kit for Illumina	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers	NEBNext Ultra II FS DNA Library Prep	DNA Sequencing on the G4^® Sequencing Platform Using the NEBNext^® Ultra II™ FS DNA Library Prep Kit

Paragon Genomics

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
CleanPlex OncoZoom Cancer Hotspot Panel CleanPlex Hereditary Cancer Panel v2	SG UDI Primers	CleanPlex NGS Panel User Guide and CleanPlex NGS Panel Addendum User Guide for Sequencing on the G4 Platform	Multiplex PCR Targeted Enrichment Solution on the G4 Sequencing Platform

Partner Product

Singular Genomics Products

Protocol

Technical Resource & Data

CleanPlex OncoZoom Cancer Hotspot Panel
CleanPlex Hereditary Cancer Panel v2

SG UDI Primers

CleanPlex NGS Panel User Guide

and

CleanPlex NGS Panel Addendum User Guide for Sequencing on the G4 Platform

Multiplex PCR Targeted Enrichment Solution on the G4 Sequencing Platform

Parse Biosciences

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
Evercode WT	SG Library Compatibility Kit	Coming Soon	Single Cell Sequencing Performance Using the Parse Biosciences Evercode™ WT kit and the G4® Sequencing Platform

Phase Genomics

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
Proximo Hi-C Kit	SG Universal Library Prep Adapter + UDI Primers	Adapt the Phase Genomics Proximo Hi-C Kit for sequencing on the G4	Coming Soon

QIAGEN

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
QIAseq miRNA Library Kit	SG UDI Primers	QIAseq miRNA Library Kit Handbook for Singular Genomics G4 Sequencing Platform	Next-Generation Sequencing Evaluation of QIAseq miRNA-derived libraries on the Singular Genomics G4® Sequencing Platform

QuantaBio

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
sparQ DNA Library Prep Kit	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers	Quantabio sparQ DNA Library Prep	Performance Evaluation of the G4® Sequencing Platform for Microbiome Community Analysis
sparQ DNA Library Prep Kit	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers	Quantabio sparQ DNA Library Prep	Human Genome Sequencing Technical Report
sparQ DNA Frag & Library Prep Kit	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers	Quantabio sparQ DNA Library Prep	Whole Exome Sequencing on the G4®
sparQ RNA-Seq HMR Kit	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers	Quantabio sparQ RNA-Seq HMR Kit	Streamlined Whole Transcriptome Sequencing Using Singular Genomics G4® Platform and Quantabio sparQ RNA-Seq HMR Kit

Roche

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
Kapa HyperPrep PCR-free	SG Universal Library Prep Adapter + UDI Primers	KAPA EvoPlus Kit Library Preparation Workflow for Sequencing on the G4 Sequencing Platform from Singular Genomics	Rapid Whole Genome and Whole Exome Variant Detection Using a Novel Fluorescently Labeled Reversible Terminated Nucleotide Sequencing System and GPU-based Accelerated Analysis

Takara Bio

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
SMART-Seq mRNA LP	SG UDI Primers	Takara SMART-Seq mRNA LP	Discover more and maximize lab efficiency without sacrificing data quality
SMART-Seq Single Cell PLUS Kit	SG UDI Primers	SMART-Seq mRNA Single Cell LP	Discover more and maximize lab efficiency without sacrificing data quality

Tecan

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
Revelo DNA-Seq Enz for MagicPrep NGS	Not Applicable	Revelo DNA-Seq Enz for MagicPrep NGS (Singular Genomics)	MagicPrep NGS Automated Library Preparation for Sequencing on the Singular Genomics G4 Platform
Revelo DNA-Seq Mech for Magic Prep NGS	Not Applicable	Revelo DNA-Seq Mech for MagicPrep NGS (Singular Genomics)	MagicPrep NGS Automated Library Preparation for Sequencing on the Singular Genomics G4 Platform
Revelo mRNA-Seq for MagicPrep NGS	Not Applicable	Revelo mRNA-Seq for MagicPrep NGS (Singular Genomics)	MagicPrep NGS Automated Library Preparation for Sequencing on the Singular Genomics G4 Platform

Watchmaker Genomics

Partner Product	Singular Genomics Products	Protocol	Technical Resource & Data
Watchmaker Genomics DNA Library Prep Kit	SG Universal Library Prep Adapter + UDI Primers	Watchmaker DNA Library Prep Kit with Fragmentation for library construction on the Singular G4™ Platform	Scalable and Flexible Library Preparation for High Sensitivity Applications

10X Genomics

Partner Product	Chromium Single Cell Visium Spatial Gene Expression (FFPE and Fresh Frozen)
Singular Genomics Products	SG t-UDI Primers Set A [1–24] SG Non-Indexed Library Prep t-Primers
Protocol	10x Genomics Library Prep
Technical Resource & Data	G4™ Single Cell RNA-Sequencing Performance with the 10x Genomics® Chromium Single Cell 3’ Gene Expression Kit

Partner Product	Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 Chromium Next GEM Single Cell 5’ Reagent Kits v2 Visium Spatial Gene Expression Reagent Kits Visium Spatial Gene Expression Reagent Kits for FFPE
Singular Genomics Products	Max Read Single Cell and Spatial Gene Expression Max Read Spatial Gene Expression for FFPE
Protocol	10x Genomics Library Prep
Technical Resource & Data	The Max Read™ Kit for High-Throughput Single Cell Sequencing on the G4®

Agilent

Partner Product	SureSelect Exome v8 and XT HS2 Kits
Singular Genomics Products	SG Library Compatibility Kit
Protocol	Adapting Libraries for the G4™—Retaining Original Indices
Technical Resource & Data	Targeted Sequencing Using Agilent SureSelect and the G4™ Sequencing Platform

Bio-Rad

Partner Product	SEQuoia Express Stranded RNA Library Preparation Kit SEQuoia Complete Stranded RNA Library Preparation Kit SEQuoia RiboDepletion Kit, 24 reactions
Singular Genomics Products	SG UDI Primers
Protocol	Bio-Rad SEQuoia Stranded RNA Library Prep
Technical Resource & Data	RNA Sequencing on the Singular Genomics G4™ Sequencing Platform Using SEQuoia™ Library Preparation Chemistries

Claret

Partner Product	SRSLY PicoPlus ForShearSRSLY NanoPlus
Singular Genomics Products	SG UDI Primers SG Non-Indexed Library Prep Primers
Protocol	Claret SRSLY Library Prep
Technical Resource & Data	Sequence SRSLY® Libraries on the Singular Genomics G4™ Platform

Fluent Biosciences

Partner Product	PIPseq 3’ RNA kit
Singular Genomics Products	Order custom PCR and sequencing primers
Protocol	Claret Sequencing PIPseq with Singular Genomics Demonstrated Protocol
Technical Resource & Data	PIPseq Compatibility with the Singular Genomics G4 Sequencing Platform

IDT

Partner Product	IDT Blockers
Singular Genomics Products	Not Applicable
Protocol	Singular Genomics-specific blockers
Technical Resource & Data	Whole Exome Sequencing on the G4™

Partner Product	xGen cfDNA & FFPE DNA Library Prep Kit
Singular Genomics Products	SG UDI Primers
Protocol	xGen™ cfDNA & FFPE DNA Library Prep Kit for the Singular Genomics G4® Sequencing Platform
Technical Resource & Data	Low-Copy Number Variant Detection Using xGen™ NGS DNA Library Prep and G4® Sequencing Platform

Partner Product	xGen Hybridization and Wash Kit with xGen Exome Hyb Panel v2
Singular Genomics Products	SG UDI Primers SG Non-Indexed Library Prep Primers
Protocol
Technical Resource & Data	Whole Exome Sequencing on the G4®

Partner Product	xGen cfDNA kit with xGen Pan Cancer Panel
Singular Genomics Products	Not Applicable
Protocol	Coming Soon
Technical Resource & Data	Rapid Targeted Germline and Somatic Variant Detection Using the G4® Sequencing Platform

NEB

Partner Product	NEBNext Enzymatic Methyl-seq Kit
Singular Genomics Products	SG UDI Primers SG Non-Indexed Library Prep Primers
Protocol	Coming Soon
Technical Resource & Data	Performance Assessment of the Novel G4® Sequencing Platform for Cancer Research

Partner Product	NEBNext Ultra II Directional RNA Library Prep Kit for Illumina RNA Depletion Kit v2 (Human/ Mouse/ Rat)
Singular Genomics Products	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers
Protocol	NEBNext Ultra II Directional RNA Library Prep
Technical Resource & Data	RNA Sequencing Using the New England Biolabs (NEB) Ultra ll RNA Library Prep Kit and the G4® Sequencing Platform

Partner Product	NEBNext Ultra II FS DNA Library Prep Kit for Illumina
Singular Genomics Products	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers
Protocol	NEBNext Ultra II FS DNA Library Prep
Technical Resource & Data	DNA Sequencing on the G4^® Sequencing Platform Using the NEBNext^® Ultra II™ FS DNA Library Prep Kit

Paragon

Partner Product	CleanPlex OncoZoom Cancer Hotspot Panel CleanPlex Hereditary Cancer Panel v2
Singular Genomics Products	SG UDI Primers
Protocol	CleanPlex NGS Panel User Guide and CleanPlex NGS Panel Addendum User Guide for Sequencing on the G4 Platform
Technical Resource & Data	Multiplex PCR Targeted Enrichment Solution on the G4 Sequencing Platform

Parse

Partner Product	Evercode WT
Singular Genomics Products	SG Library Compatibility Kit
Protocol	Coming Soon
Technical Resource & Data	Single Cell Sequencing Performance Using the Parse Biosciences Evercode™ WT kit and the G4® Sequencing Platform

Phase

Partner Product	Proximo Hi-C Kit
Singular Genomics Products	SG Universal Library Prep Adapter + UDI Primers
Protocol	Adapt the Phase Genomics Proximo Hi-C Kit for sequencing on the G4
Technical Resource & Data	Coming Soon

Qiagen

Partner Product	QIAseq miRNA Library Kit
Singular Genomics Products	SG UDI Primers
Protocol	QIAseq miRNA Library Kit Handbook for Singular Genomics G4 Sequencing Platform
Technical Resource & Data	Next-Generation Sequencing Evaluation of QIAseq miRNA-derived libraries on the Singular Genomics G4® Sequencing Platform

Quantabio

Partner Product	sparQ DNA Library Prep Kit
Singular Genomics Products	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers
Protocol	Quantabio sparQ DNA Library Prep
Technical Resource & Data	Performance Evaluation of the G4® Sequencing Platform for Microbiome Community Analysis
Technical Resource & Data	Human Genome Sequencing Technical Report

Partner Product	sparQ DNA Frag & Library Prep Kit
Singular Genomics Products	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers
Protocol	Quantabio sparQ DNA Library Prep
Technical Resource & Data	Whole Exome Sequencing on the G4®

Partner Product	sparQ RNA-Seq HMR Kit
Singular Genomics Products	SG Universal Library Prep Adapter + UDI Primers SG Universal Library Prep Adapter + SG Non-Indexed Library Prep Primers
Protocol	Quantabio sparQ RNA-Seq HMR Kit
Technical Resource & Data	Streamlined Whole Transcriptome Sequencing Using Singular Genomics G4® Platform and Quantabio sparQ RNA-Seq HMR Kit

Roche

Partner Product	Kapa HyperPrep PCR-free
Singular Genomics Products	SG Universal Library Prep Adapter + UDI Primers
Protocol	KAPA EvoPlus Kit Library Preparation Workflow for Sequencing on the G4 Sequencing Platform from Singular Genomics
Technical Resource & Data	Rapid Whole Genome and Whole Exome Variant Detection Using a Novel Fluorescently Labeled Reversible Terminated Nucleotide Sequencing System and GPU-based Accelerated Analysis

Takara

Partner Product	SMART-Seq mRNA LP
Singular Genomics Products	SG UDI Primers
Protocol	Coming Soon
Technical Resource & Data	Discover more and maximize lab efficiency without sacrificing data quality

Partner Product	SMART-Seq Single Cell PLUS Kit
Singular Genomics Products	SG UDI Primers
Protocol	Revelo mRNA-Seq for MagicPrep NGS (Singular Genomics)
Technical Resource & Data	Discover more and maximize lab efficiency without sacrificing data quality

Tecan

Partner Product	Revelo DNA-Seq Enz for MagicPrep NGS
Singular Genomics Products	Not Applicable
Protocol	Revelo DNA-Seq Enz for MagicPrep NGS (Singular Genomics)
Technical Resource & Data	MagicPrepTM NGS Automated Library Preparation for Sequencing on the Singular Genomics G4TM Platform

Partner Product	Revelo DNA-Seq Mech for Magic Prep NGS
Singular Genomics Products	Not Applicable
Protocol	Revelo DNA-Seq Mech for MagicPrep NGS (Singular Genomics)
Technical Resource & Data	MagicPrep NGS Automated Library Preparation for Sequencing on the Singular Genomics G4 Platform

Partner Product	Revelo mRNA-Seq for MagicPrep NGS
Singular Genomics Products	Not Applicable
Protocol	Revelo mRNA-Seq for MagicPrep NGS (Singular Genomics)
Technical Resource & Data	MagicPrep NGS Automated Library Preparation for Sequencing on the Singular Genomics G4 Platform

Watchmaker

Partner Product	Watchmaker Genomics DNA Library Prep Kit
Singular Genomics Products	SG Universal Library Prep Adapter + UDI Primers
Protocol	Watchmaker DNA Library Prep Kit with Fragmentation for library construction on the Singular G4™ Platform
Technical Resource & Data	Scalable and Flexible Library Preparation for High Sensitivity Applications

G4 FAQs

Preparation FAQs

What are the sequences of Singular adapters?

What is the structure of Singular adapters?

Does the G4 Sequencing Platform support indexed libraries?

Sequencing FAQs

What is the format for the G4 Sample Sheet?

What is the Q30 performance for the G4 Sequencing Platform?

Does the G4 Sequencing Platform support paired reads (paired-end) sequencing?

Analysis FAQs

What data format does the G4 Sequencing Platform output?

G4 Support Videos

G4 Overview

Preparing for a Run

Sample Loading

Disposal of Reagent Cartridge

Handling and Loading Flow Cells

Reagent Cartridges

Custom Primers

Sample Preparation

Water Disposal

Demo Data

For FASTQ files and data generated on the G4 Sequencing Platform, please contact our customer care team. The following datasets are available:

Whole Genome Sequencing
RNA Sequencing
Single Cell RNA Sequencing
Whole Exome Sequencing

Tools

Loading Concentration Calculator

Calculate the various denature, dilute, and pooling steps of your libraries before you load them on the instrument.

Sample Sheet Generator

Set up your sample data sheet and check the data for errors and incompatibilities.

Site Readiness

Review the site requirements needed to ensure that your laboratory is prepared for G4 installation, validation, and sequencing.

Meet the Team

Our team is devoted to supporting your science. Field application scientists, field service engineers, and customer care specialists are dedicated to responding quickly and effectively to your inquiries.